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Lected and disrupted by sonication in lysis buffer (25 mM Tris, pH 7.8, 2 mM EDTA, 1 Triton X-100, and 10 glycerol). After centrifugation, aliquots of the supernatants were tested for luciferase activity using the luciferase assay system. Firefly luciferase activities were standardized for b-galactosidase activity.To detect the in vivo association of nuclear proteins with rat mmp-9 promoter, chr
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Lected and disrupted by sonication in lysis buffer (25 mM Tris, pH 7.8, 2 mM EDTA, 1 Triton X-100, and 10 glycerol). After centrifugation, aliquots of the supernatants were tested for luciferase activity using the luciferase assay system. Firefly luciferase activities were standardized for b-galactosidase activity.To detect the in vivo association of nuclear proteins with rat mmp-9 promoter, chr
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Awaiting a lung transplant every year (3-5). The resulting estimates ofAwaiting a lung transplant every year (3-5). The resulting estimates of mortality for patients on the waitlist is concerning, and has raised considerable interest in looking for alternative bridging strategies for patients with end-stage lung disease awaiting transplantation (2).?Pioneer Bioscience Publishing Company. All
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https://electronics.howstuffworks.com/how-to-tech/how-to-reduce-technology-carbon-footprint.htmThe word "percolator" has come to mean that special coffee pot that used to sit on Grandma's stove and bubble away like mad all morning long. Typically they consist of a heat proof pot that holds the water, a long tube (like a straw) that holds a filter basket at the top. This filter is norma
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http://www.prhwy.com/no/page5.htmlThe word "percolator" has come to mean that special coffee pot that used to sit on Grandma's stove and bubble away like mad all morning long. Typically they consist of a heat proof pot that holds the water, a long tube (like a straw) that holds a filter basket at the top. This filter is normally made of metal and holds the coffee grounds apart from the