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N. Roc2KG mutant animals develop normally, and we could detect no obvious phenotype, except for a slight reduction in female fecundity. The Cul5 locus is simpler than that of Roc2, and the Cul5 transcript includes 7 exons and 6 introns. We identified a P element (Cul5EY) that is inserted into the second exon of Cul5 at amino acid D346 of the predicted open reading frame (Fig. 7B). RT-PCR analysis
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